GAD Protein (AA 1-224) (His tag)
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- Target See all GAD (GAD1) Proteins
- GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-224
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GAD protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMASSTPS SSATSSNAGA DPNTTNLRPT TYDTWCGVAH GCTRKLGLKI CGFLQRTNSL EEKSRLVSAF KERQSSKNLL SCENSDRDAR FRRTETDFSN LFARDLLPAK NGEEQTVQFL LEVVDILLNY VRKTFDRSTK VLDFHHPHQL LEGMEGFNLE LSDHPESLEQ ILVDCRDTLK YGVRTGHPRF FNQLSTGLDI IGLAGEWLTS TANTNMPSDM RECWLLR
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product GAD1 Protein
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Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 1-594) protein (GST tag)
GAD1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 1-594) protein (Strep Tag)Crystallography grade GAD1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) (AA 1-593) protein (Strep Tag)Crystallography grade GAD1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) proteinGAD1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1) protein (Myc-DYKDDDDK Tag)GAD1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Denatured
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 0.4M urea
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))
- Alternative Name
- GAD1 (GAD1 Products)
- Synonyms
- CPSQ1 Protein, GAD Protein, SCP Protein, GAD-67 Protein, cpsq1 Protein, gad1 Protein, gad1-A Protein, gad67 Protein, GAD67 Protein, GAD1 Protein, GLUTAMATE DECARBOXYLASE Protein, GLUTAMATE DECARBOXYLASE 1 Protein, MKP11.30 Protein, MKP11_30 Protein, glutamate decarboxylase Protein, EP10 Protein, GAD25 Protein, GAD44 Protein, Gad-1 Protein, Gad67 Protein, glutamate decarboxylase 1 Protein, glutamate decarboxylase 1 L homeolog Protein, glutamate decarboxylase Protein, glutamate decarboxylase 1b Protein, GAD1 Protein, gad1.1.L Protein, GAD Protein, Gad1 Protein, gad1b Protein
- Background
- GAD1 is one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. It is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This protien may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. Recombinant human GAD1 protein, fused to His-tag at N-terminus, was expressed in E.coli .
- Molecular Weight
- 27.7 kDa (247 aa)
- NCBI Accession
- NP_038473
- UniProt
- Q99259
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