PURB Protein (AA 1-312) (His tag)
-
- Target See all PURB Proteins
- PURB (Purine-Rich Element Binding Protein B (PURB))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-312
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PURB protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMADGDSG SERGGGGGPC GFQPASRGGG EQETQELASK RLDIQNKRFY LDVKQNAKGR FLKIAEVGAG GSKSRLTLSM AVAAEFRDSL GDFIEHYAQL GPSSPEQLAA GAEEGGGPRR ALKSEFLVRE NRKYYLDLKE NQRGRFLRIR QTVNRGGGGF GAGPGPGGLQ SGQTIALPAQ GLIEFRDALA KLIDDYGGED DELAGGPGGG AGGPGGGLYG ELPEGTSITV DSKRFFFDVG CNKYGVFLRV SEVKPSYRNA ITVPFKAWGK FGGAFCRYAD EMKEIQERQR DKLYERRGGG SGGGEESEGE EVDED
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product PURB Protein
-
Purine-Rich Element Binding Protein B (PURB) (AA 2-312) protein (His tag)
Crystallography grade PURB Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, Crys, WB
Purine-Rich Element Binding Protein B (PURB) (AA 2-324) protein (His tag)Crystallography grade PURB Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, Crys, WB
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- PURB (Purine-Rich Element Binding Protein B (PURB))
- Alternative Name
- PuRB (PURB Products)
- Synonyms
- 2310015K15Rik Protein, AA114818 Protein, Cager-2 Protein, D11Bwg0414e Protein, pur-beta Protein, purb Protein, zgc:65916 Protein, purb-b Protein, purbeta Protein, PURBETA Protein, si:dkey-202n14.1 Protein, purine rich element binding protein B Protein, purine-rich element binding protein Bb Protein, purine-rich element binding protein B L homeolog Protein, purine-rich element binding protein Ba Protein, Purb Protein, purbb Protein, purb.L Protein, PURB Protein, purba Protein
- Background
- PuRB is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PuR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. Recombinant human PuRB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 35.6kDa (335aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_150093
- UniProt
- Q96QR8
-
