ZWILCH Protein (AA 1-591) (His tag)
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- Target See all ZWILCH Proteins
- ZWILCH (Zwilch, Kinetochore Associated, Homolog (ZWILCH))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-591
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ZWILCH protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMWERLNC AAEDFYSRLL QKFNEEKKGI RKDPFLYEAD VQVQLISKGQ PNPLKNILNE NDIVFIVEKV PLEKEETSHI EELQSEETAI SDFSTGENVG PLALPVGKAR QLIGLYTMAH NPNMTHLKIN LPVTALPPLW VRCDSSDPEG TCWLGAELIT TNNSITGIVL YVVSCKADKN YSVNLENLKN LHKKRHHLST VTSKGFAQYE LFKSSALDDT ITASQTAIAL DISWSPVDEI LQIPPLSSTA TLNIKVESGE PRGPLNHLYR ELKFLLVLAD GLRTGVTEWL EPLEAKSAVE LVQEFLNDLN KLDGFGDSTK KDTEVETLKH DTAAVDRSVK RLFKVRSDLD FAEQLWCKMS SSVISYQDLV KCFTLIIQSL QRGDIQPWLH SGSNSLLSKL IHQSYHGTMD TVSLSGTIPV QMLLEIGLDK LKKDYISFFI GQELASLNHL EYFIAPSVDI QEQVYRVQKL HHILEILVSC MPFIKSQHEL LFSLTQICIK YYKQNPLDEQ HIFQLPVRPT AVKNLYQSEK PQKWRVEIYS GQKKIKTVWQ LSDSSPIDHL NFHKPDFSEL TLNGSLEERI FFTNMVTCSQ VHFK
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product ZWILCH Protein
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Zwilch, Kinetochore Associated, Homolog (ZWILCH) (AA 1-477) protein (GST tag)
ZWILCH Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Zwilch, Kinetochore Associated, Homolog (ZWILCH) (AA 1-591) protein (Strep Tag)Crystallography grade ZWILCH Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 20 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- ZWILCH (Zwilch, Kinetochore Associated, Homolog (ZWILCH))
- Alternative Name
- ZWILCH (ZWILCH Products)
- Synonyms
- CG18639 Protein, CG18729 Protein, Dmel\\CG18729 Protein, Zwilch Protein, hzwilch Protein, kntc1ap Protein, KNTC1AP Protein, hZwilch Protein, 2310031L18Rik Protein, si:ch211-242m18.4 Protein, wu:fb10f09 Protein, zwilch kinetochore protein Protein, CG18729 gene product from transcript CG18729-RA Protein, protein zwilch homolog Protein, Zwilch Protein, zwilch Protein, LOC100374963 Protein, ZWILCH Protein
- Background
- ZWILCH belongs to the ZWILCH family. This protein is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. It is required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Recombinant human ZWILCH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 69.6kDa(614aa)
- NCBI Accession
- NP_060445
- UniProt
- Q9H900
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