GARS Protein (AA 43-289) (His tag)
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- Target See all GARS Proteins
- GARS (Glycyl-tRNA Synthetase (GARS))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 43-289
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GARS protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSPISLPAA ASRSSMDGAG AEEVLAPLRL AVRQQGDLVR KLKEDKAPQV DVDKAVAELK ARKRVLEAKE LALQPKDDIV DRAKMEDTLK RRFFYDQAFA IYGGVSGLYD FGPVGCALKN NIIQTWRQHF IQEEQILEID CTMLTPEPVL KTSGHVDKFA DFMVKDVKNG ECFRADHLLK AHLQKLMSDK KCSVEKKSEM ESVLAQLDNY GQQELADLFV NYNVKSPITG NDLSPPVSFN LMFKTFIGPG
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product GARS Protein
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Glycyl-tRNA Synthetase (GARS) (AA 531-739) protein (His-IF2DI Tag)
GARS Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Glycyl-tRNA Synthetase (GARS) (AA 1-685) protein (GST tag)GARS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glycyl-tRNA Synthetase (GARS) (AA 1-739) protein (His tag)Crystallography grade GARS Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glycyl-tRNA Synthetase (GARS) (AA 1-729) protein (His tag)Crystallography grade GARS Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glycyl-tRNA Synthetase (GARS) protein (Myc-DYKDDDDK Tag)GARS Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- GARS (Glycyl-tRNA Synthetase (GARS))
- Alternative Name
- GARS (GARS Products)
- Synonyms
- CMT2D Protein, DSMAV Protein, GlyRS Protein, HMN5 Protein, SMAD1 Protein, Aat-gly Protein, CG6778 Protein, Dmel\\CG6778 Protein, GRS Protein, gars Protein, team Protein, RGD1559871 Protein, GENA202 Protein, Gena201 Protein, Nmf249 Protein, Sgrp23 Protein, GB13467 Protein, MGC79495 Protein, fb02f03 Protein, si:dkey-276i5.1 Protein, wu:fb02f03 Protein, NI36_08435 Protein, glycyl-tRNA synthetase Protein, Glycyl-tRNA synthetase Protein, glycine--tRNA ligase Protein, glycyl-tRNA synthetase L homeolog Protein, Glycine--tRNA ligase Protein, GARS Protein, GlyRS Protein, Gars Protein, LOC408392 Protein, gars.L Protein, gars Protein, LOC692991 Protein, glyQ Protein, TTHA0543 Protein, NI36_RS08405 Protein
- Background
- GARS, also known as glycyl-tRNA synthetase, is one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Recombinant human GARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 30.0 kDa (270aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_002038
- UniProt
- P41250
- Pathways
- Ribonucleoside Biosynthetic Process
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