MICA Protein (AA 24-297) (His tag)
Quick Overview for MICA Protein (AA 24-297) (His tag) (ABIN7280944)
Target
See all MICA ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 24-297
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Purification tag / Conjugate
- This MICA protein is labelled with His tag.
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Sequence
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MEPHSLRYNL TVLSWDGSVQ SGFLTEVHLD GQPFLRCDRQ KCRAKPQGQW AEDVLGNKTW DRETRDLTGN GKDLRMTLAH IKDQKEGLHS LQEIRVCEIH EDNSTRSSQH FYYDGELFLS QNLETEEWTM PQSSRAQTLA MNVRNFLKED AMKTKTHYHA MHADCLQELR RYLKSGVVLR RTVPPMVNVT RSEASEGNIT VTCRASGFYP WNITLSWRQD GVSLSHDTQQ WGDVLPDGNG TYQTWVATRI CQGEEQRFTC YMEHSGNHST HPVPS
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Purification
- > 90% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA with Human NKG2D (CAT# ATGP3662)
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MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-308) (Active) protein (Biotin,His-Avi Tag)
MICA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 1-284) (Active) protein (His tag)MICA Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-308) (Active) protein (His-Avi Tag)MICA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-307) protein (His tag)MICA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-308) (Active) protein (Fc Tag)MICA Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 1-383) protein (GST tag)MICA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-307) protein (His tag)MICA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
MHC Class I Polypeptide-Related Sequence A (MICA) (AA 24-306) protein (His-IF2DI Tag)MICA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 10 % Glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
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Alternative Name
- MICA
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Background
- MICA also known as MHC class I polypeptide-related sequence A. This Protein's Function seems to have no role in antigen presentation. It acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. It binding to KLRK1 leads to cell lysis. Recombinant human MICA, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 32.7 kDa (283aa) confirmed by MALDI-TOF
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Pathways
- Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response
Target
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