MICA Protein (AA 24-308) (His-Avi Tag)

Catalog No. ABIN7275255

This Recombinant MICA protein is produced in HEK-293 Cells.

Quick Overview for MICA Protein (AA 24-308) (His-Avi Tag) (ABIN7275255)

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-308

Purification tag / Conjugate: This MICA protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human MICA Protein

Sequence: Glu24-Gln308

Characteristics: Recombinant Human MICA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu24-Gln308.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human MICA, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).,Immobilized Human MICA, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MICA Antibody, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA.,Human MICA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human MICA is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Alternative Name: MICA

Background: MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85 % amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..

Molecular Weight: 35.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

UniProt: Q96QC4

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response