PYCR2 Protein (AA 1-320) (His tag)
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- Target See all PYCR2 Proteins
- PYCR2 (Pyrroline-5-Carboxylate Reductase Family, Member 2 (PYCR2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-320
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PYCR2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMSVGFIG AGQLAYALAR GFTAAGILSA HKIIASSPEM NLPTVSALRK MGVNLTRSNK ETVKHSDVLF LAVKPHIIPF ILDEIGADVQ ARHIVVSCAA GVTISSVEKK LMAFQPAPKV IRCMTNTPVV VQEGATVYAT GTHALVEDGQ LLEQLMSSVG FCTEVEEDLI DAVTGLSGSG PAYAFMALDA LADGGVKMGL PRRLAIQLGA QALLGAAKML LDSEQHPCQL KDNVCSPGGA TIHALHFLES GGFRSLLINA VEASCIRTRE LQSMADQEKI SPAALKKTLL DRVKLESPTV STLTPSSPGK LLTRSLALGG KKD
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product PYCR2 Protein
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Pyrroline-5-Carboxylate Reductase Family, Member 2 (PYCR2) (AA 263-320) protein (His-IF2DI Tag)
PYCR2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Pyrroline-5-Carboxylate Reductase Family, Member 2 (PYCR2) (AA 1-320) protein (GST tag)PYCR2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Pyrroline-5-Carboxylate Reductase Family, Member 2 (PYCR2) (AA 2-320) protein (His tag)Crystallography grade PYCR2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Pyrroline-5-Carboxylate Reductase Family, Member 2 (PYCR2) (AA 2-320) protein (His tag)Crystallography grade PYCR2 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Pyrroline-5-Carboxylate Reductase Family, Member 2 (PYCR2) protein (Myc-DYKDDDDK Tag)PYCR2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In phosphate buffered saline ( pH 7.4) containing 50 % glycerol, 5 mM DTT, 1 mM EDTA.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PYCR2 (Pyrroline-5-Carboxylate Reductase Family, Member 2 (PYCR2))
- Alternative Name
- PYCR2 (PYCR2 Products)
- Synonyms
- p5c Protein, p5cr Protein, pro3 Protein, pycr Protein, pig45 Protein, pp222 Protein, pycr2 Protein, arcl2b Protein, P5CR2 Protein, 1810018M05Rik Protein, P5cr2 Protein, pyrroline-5-carboxylate reductase 2 Protein, pyrroline-5-carboxylate reductase family, member 1 Protein, pyrroline-5-carboxylate reductase 1 Protein, pyrroline-5-carboxylate reductase family, member 2 Protein, pyrroline-5-carboxylate reductase 1b Protein, PYCR2 Protein, pycr1 Protein, PYCR1 Protein, Pycr2 Protein, pycr1b Protein
- Background
- PYCR2 also known as pyrroline-5-carboxylate reductase 2 isoform 1, belongs to the pyrroline-5-carboxylate reductase family. This protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. The 3 substrates of this enzyme are L-proline, NAD+, and NADP+, whereas its 4 products are 1-pyrroline-5-carboxylate, NADH, NADPH, and H+. Recombinant human PYCR2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 36kDa (343aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_037460
- UniProt
- Q96C36
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