DCT Protein (AA 24-472) (His tag)

Catalog No. ABIN5854719

Product Details

Target: DCT (Dopachrome Tautomerase (DCT))

Protein Type: Recombinant

Protein Characteristics: AA 24-472

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This DCT protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: QFPRVCMTVD SLVNKECCPR LGAESANVCG SQQGRGQCTE VRADTRPWSG PYILRNQDDR ELWPRKFFHR TCKCTGNFAG YNCGDCKFGW TGPNCERKKP PVIRQNIHSL SPQEREQFLG ALDLAKKRVH PDYVITTQHW LGLLGPNGTQ PQFANCSVYD FFVWLHYYSV RDTLLGPGRP YRAIDFSHQG PAFVTWHRYH LLCLERDLQR LIGNESFALP YWNFATGRNE CDVCTDQLFG AARPDDPTLI SRNSRFSSWE TVCDSLDDYN HLVTLCNGTY EGLLRRNQMG RNSMKLPTLK DIRDCLSLQK FDNPPFFQNS TFSFRNALEG FDKADGTLDS QVMSLHNLVH SFLNGTNALP HSAANDPIFV VLHSFTDAIF DEWMKRFNPP ADAWPQELAP IGHNRMYNMV PFFPPVTNEE LFLTSDQLGY SYAIDLPVSV EETPGWPTTH HHHHH

Purity: > 90 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: DCT (Dopachrome Tautomerase (DCT))

Alternative Name: DCT (DCT Products)

Synonyms: TYRP2 Protein, RGD1564975 Protein, trp2 Protein, DT Protein, TRP-2 Protein, TRP2 Protein, Tyrp-2 Protein, Tyrp2 Protein, slaty Protein, slt Protein, zgc:109860 Protein, dopachrome tautomerase Protein, dopachrome tautomerase L homeolog Protein, DCT Protein, Dct Protein, dct Protein, dct.L Protein

Background: DCT, as known as L-dopachrome tautomerase isoform 1, is the tyrosinase family members. This protein plays a key role in the synthesis of the melanin pigment including tyrosinase (Tyr), tyrosinase-related protein 1 (Tyrp1). These are strongly implicated in eumelanin synthesis. The switch between the eumelanin and the pheomelanin pathways is thought to depend on the presence of cysteine. Thus, in the absence of cysteine, dopaquinone, the product of tyrosinase action, is transformed to cyclodopa (leucodopachrome) and then to dopachrome (and DOPA). Recombinant human DCT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 52.1kDa (455aa) 50-70kDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_001913

UniProt: P40126