2B4 Protein (AA 19-224) (hIgG-His-tag)

Catalog No. ABIN7281222

Recombinant 2B4 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for 2B4 Protein (AA 19-224) (hIgG-His-tag) (ABIN7281222)

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS), Activity Assay (AcA)

Purity: > 90 %

Product Details

Protein Characteristics: AA 19-224

Purification tag / Conjugate: This 2B4 protein is labelled with hIgG-His-tag.

Sequence: GKGCQGS ADHVVSISGV PLQLQPNSIQ TKVDSIAWKK LLPSQNGFHH ILKWENGSLP SNTSNDRFSF IVKNLSLLIK AAQQQDSGLY CLEVTSISGK VQTATFQVFV FDKVEKPRLQ GQGKILDRGR CQVALSCLVS RDGNVSYAWY RGSKLIQTAG NLTYLDEEVD INGTHTYTCN VSNPVSWESH TLNLTQDCQN AHQEFRFWP

Purification: > 90% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured by its binding ability in a functional ELISA with Human CD48/SLAMF2 (CAT# ATGP3642). The ED50 range ≤ 0.1 ug/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: 2B4/CD244

Background: CD244, also known as natural killer cell receptor 2B4 isoform 1, is a natural killer and T cell immunoglobulin superfamily surface protein and ligand of CD48. It is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. It is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. It activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness. Recombinant human CD244, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 50.2 kDa (448aa)

NCBI Accession: NP_057466