Thymic Stromal Lymphopoietin Protein (TSLP) (AA 29-159) (His tag,AVI tag,Biotin)

Catalog No. ABIN7457999

This Recombinant Thymic Stromal Lymphopoietin protein is produced in HEK-293 Cells.

Quick Overview for Thymic Stromal Lymphopoietin Protein (TSLP) (AA 29-159) (His tag,AVI tag,Biotin) (ABIN7457999)

Target: Thymic Stromal Lymphopoietin (TSLP)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >85 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 29-159

Purification tag / Conjugate: This Thymic Stromal Lymphopoietin protein is labelled with His tag,AVI tag,Biotin.

Sequence: AA 29-159

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP

Background: Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.

Molecular Weight: 18.6 kDa