Thymic Stromal Lymphopoietin Protein (TSLP) (AA 29-159) (Fc Tag)

Catalog No. ABIN7092793

This Recombinant Thymic Stromal Lymphopoietin protein is produced in HEK-293 Cells.

Quick Overview for Thymic Stromal Lymphopoietin Protein (TSLP) (AA 29-159) (Fc Tag) (ABIN7092793)

Target: Thymic Stromal Lymphopoietin (TSLP)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Drug Assay (ADA), Affinity Measurement (AM), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 29-159

Purification tag / Conjugate: This Thymic Stromal Lymphopoietin protein is labelled with Fc Tag.

Purpose: Recombinant human TSLP protein with C-terminal human Fc tag

Specificity: TSLP(Tyr29-Gln159) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP

Background: TSLP
This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c( ) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. In addition, the shorter (predominant) isoform is an antimicrobial protein, displaying antibacterial and antifungal activity against B. cereus, E. coli, E. faecalis, S. mitis, S. epidermidis, and C. albicans. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2020]

Molecular Weight: predicted molecular mass of 41.1 kDa after removal of the signal peptide. The apparent molecular mass of TSLP-hFc is 55-70 kDa due to glycosylation.

UniProt: Q969D9