Thymic Stromal Lymphopoietin Protein (TSLP) (AA 29-159, R127A, R130A) (His-Avi Tag)

Catalog No. ABIN7275807

This Recombinant Thymic Stromal Lymphopoietin protein is produced in HEK-293 Cells.

Quick Overview for Thymic Stromal Lymphopoietin Protein (TSLP) (AA 29-159, R127A, R130A) (His-Avi Tag) (ABIN7275807)

Target: Thymic Stromal Lymphopoietin (TSLP)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 29-159, R127A, R130A

Purification tag / Conjugate: This Thymic Stromal Lymphopoietin protein is labelled with His-Avi Tag.

Purpose: Human TSLP (R127A, R130A) Protein

Sequence: Tyr29-Gln159(R127A,R130A)

Characteristics: Recombinant Human TSLP (R127A, R130A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159(R127A,R130A).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TSLP (R127A, R130A) , His Tag at 1μg/ml (100μl/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP

Background: Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Molecular Weight: 17.7 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Tris-Bis PAGE result.

UniProt: Q969D9-1