Leptin Protein (LEP) (His tag)
Quick Overview for Leptin Protein (LEP) (His tag) (ABIN6124311)
Target
See all Leptin (LEP) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Leptin protein is labelled with His tag.
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Purpose
- Dog Leptin (LEP) Protein
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Sequence
- VPIRKVQDD TKTLIKTIVA RINDISHTQS VSSKQRVAGL DFIPGLQPVL SLSRMDQTLA IYQQILNSLH SRNVVQISND LENLRDLLHL LASSKSCPLP RARGLETFES LGGVLEASLY STEVVALNRL QAALQDMLRR LDLSPGC
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Endotoxin Level
- < 1.0 EU per 1 μg (determined by the LAL method)
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Biological Activity Comment
- Not tested
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Leptin (LEP) (AA 22-167) (Active) protein (hFc Tag)
LEP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Leptin (LEP) (AA 22-167) protein (His-IF2DI Tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origin: Pig Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 428-635) protein (His-IF2DI Tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (Fc Tag)LEP Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Leptin (LEP) (AA 22-171) protein (His tag)LEP Origin: Crucian Carp Host: Yeast (Pichia pastoris) Recombinant > 95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 22-167) protein (GST tag)LEP Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Leptin (LEP) (AA 22-167) (Active) proteinLEP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. ELISA, BLI Active
Leptin (LEP) (AA 22-167), (full length) protein (mFc Tag)LEP Origin: Pig Host: Mammalian Cells Recombinant Greater than 95 % as determined by SDS-PAGE.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01 % Sarcosyl, 5 % Trehalose.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- Leptin (LEP)
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Alternative Name
- Leptin
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Molecular Weight
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Calculated MW: 19.8 kDa
Observed MW (SDS-PAGE): 20 kDa -
UniProt
- O02720
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Pathways
- JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
Target
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