Leptin Protein (LEP) (AA 428-635) (His-IF2DI Tag)
Quick Overview for Leptin Protein (LEP) (AA 428-635) (His-IF2DI Tag) (ABIN7123743)
Target
See all Leptin (LEP) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 428-635
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Purification tag / Conjugate
- This Leptin protein is labelled with His-IF2DI Tag.
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Purpose
- Recombinant Human Leptin
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Sterility
- 0.2 μm filtered
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Leptin (LEP) (AA 22-167) (Active) protein (hFc Tag)
LEP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Leptin (LEP) proteinLEP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by reducing SDS-PAGE.
Leptin (LEP) protein (His tag)LEP Origin: Goldfish Host: Yeast Recombinant > 95 % as determined by reducing SDS-PAGE.
Leptin (LEP) (AA 22-167) protein (Fc Tag)LEP Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (His tag)LEP Origin: Pig Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (His-IF2DI Tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Leptin (LEP) (AA 22-167) protein (GST tag)LEP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Leptin (LEP) proteinLEP Origin: Rat Host: Escherichia coli (E. coli) Recombinant 95,00% SDS
Leptin (LEP) (AA 29-164), (partial) protein (GST tag)LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.
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Buffer
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
- The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
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Expiry Date
- 12 months
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- Leptin (LEP)
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Alternative Name
- Leptin
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Background
- FLJ94114, LEP, LEP_HUMAN, LEPD, Leptin, Leptin (murine obesity homolog), Leptin (obesity homolog, mouse), Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, OB, Obese protein, Obese, mouse, homolog of, Obesity, Obesity factor, Obesity homolog mouse, Obesity Murine Homolog Leptin, OBS, OTTHUMP00000212285
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Molecular Weight
- 22.8 kDa
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UniProt
- P48357
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Pathways
- JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
Target
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