IL17B Protein (AA 21-180) (His tag)
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- Target See all IL17B Proteins
- IL17B (Interleukin 17B (IL17B))
- Biological Activity
- Active
- Protein Characteristics
- AA 21-180
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IL17B protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 95 %
- Biological Activity Comment
- IL17B (Interleukin-17B) is is a T cell-derived cytokine that shares sequence similarity with IL17, which has been proven to mediate potent inflammatory immune response. Besides, increasing evidence suggests that IL17 may play a critical role in various kinds of liver diseases, including hepatocellualar carcinoma. Thus a stimulation assay has been conducted to detect the bioactivity of IL17B. HepG2 cells were seeded into 24-well plate at a density of 1x105 cells/mL, and allowed to attach overnight before treated with or without certain concentrations (250ng/mL, 500ng/mL) of IL17B for 20h and IL-8 levels in the cell supernatant were determined by ELISA. Table 1. IL-8 levels in the cell supernatant of HepG2 cells up-regulated by IIL17B. Sample Concentration of IL-6 O.D. value Corrected (cell supernatant of A549 cells) (ng/mL) stimulated with IL17B (250ng/mL) 0.83 0.80 1.19 stimulated with IL17B (500ng/mL) 0.94 0.91 1.36 unstimulated 0.56 0.53 0.80 Figure 1. IL-8 levels in the cell supernatant of HepG2 cells up-regulated by IIL17B.
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Interleukin 17B (IL17B) (AA 21-180) protein (His tag)
IL17B Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
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Interleukin 17B (IL17B) (AA 1-180) protein (GST tag)IL17B Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 17B (IL17B) (AA 21-180) protein (His tag)Crystallography grade IL17B Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 17B (IL17B) (AA 23-180) protein (His tag)Crystallography grade IL17B Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Isoelectric Point: 9.7
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- IL17B (Interleukin 17B (IL17B))
- Abstract
- IL17B Products
- Synonyms
- il-17b Protein, il-20 Protein, zcyto7 Protein, IL17B Protein, IL-17B Protein, IL-20 Protein, NIRF Protein, ZCYTO7 Protein, 1110006O16Rik Protein, 1700006N07Rik Protein, Zcyto7 Protein, interleukin 17B Protein, interleukin 17B L homeolog Protein, IL17B Protein, il17b Protein, il17b.L Protein, Il17b Protein
- Background
- Alternative Names: IL-20, Cytokine Zcyto7, Neuronal interleukin-17-related factor
- Molecular Weight
- 20kDa
- UniProt
- Q9UHF5
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