MBL2 Protein (AA 130-248) (His tag,GST tag)

Catalog No. ABIN6239795

Product Details

Target: MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))

Biological Activity: Active

Protein Characteristics: AA 130-248

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MBL2 protein is labelled with His tag,GST tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His and GST Tag

Purity: > 97 %

Biological Activity Comment: MBL2 (Mannose-binding protein C) is a calcium-dependent lectin involved in innate immune defense, which binds mannose, fucose and N-acetylglucosamine on different microorganisms, therefore results in activation of the lectin pathway of the complement system. It has been proven that MASP-2 (Mannan-binding lectin serine protease 2) forms complexes with the pattern recognition molecules MBL2, triggers the activation of the complement system. Thus, a functional binding ELISA assay was constructed to detect the association of rhMBL2 with MASP2. Briefly, rhMBL2 were diluted serially in 10mM Tris-HCl, 1M NaCl, 5mM CaCl2, and 0.05%Triton X-100 (pH 7.4). Duplicate samples of 100uL were then transferred to MASP2-coated microtiter wells and incubated for 2h at 37oC. Wells were washed with PBST and incubated for 1h with anti-MBL2 mAb, then aspirated and washed 3 times. After incubation with HRP labeled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated for 15-25 minutes at 37oC. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of MBL2 with MASP2 was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of MBL2 with MASP2.

Application Details

Application Notes: Isoelectric Point: 5.8

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))

Alternative Name: Mannose Binding Lectin (MBL) (MBL2 Products)

Synonyms: COLEC1 Protein, HSMBPC Protein, MBL Protein, MBL2D Protein, MBP Protein, MBP-C Protein, MBP1 Protein, MBPD Protein, pMBP-27 Protein, mbl2 Protein, MBL1 Protein, cMBl Protein, collectin Protein, Ab2-001 Protein, Ab2-011 Protein, L-MBP Protein, MBL-C Protein, COLEC2 Protein, mbl Protein, etID42583.2 Protein, fb68b07 Protein, hbl3 Protein, wu:fb68b07 Protein, zgc:109836 Protein, mannose binding lectin 2 Protein, Mannose-binding protein C Protein, mannose-binding lectin (protein C) 2, soluble Protein, mannose-binding lectin (protein C) 2 Protein, mannose-binding lectin family member 3, pseudogene Protein, MBL2 Protein, mbl2 Protein, Mbl2 Protein, MBL3P Protein

Background: Alternative Names: MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, Mannan Binding Protein, Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)

Molecular Weight: 43kDa

UniProt: P11226

Pathways: Complement System, Positive Regulation of Immune Effector Process