NUCB2 Protein (AA 26-106) (His tag,GST tag)

Catalog No. ABIN6239855

Product Details

Target: NUCB2 (NES1) (Nesfatin-1 (NES1))

Biological Activity: Active

Protein Characteristics: AA 26-106

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NUCB2 protein is labelled with His tag,GST tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His and GST Tag

Purity: > 97 %

Biological Activity Comment: Nucb2 (Nucleobindin-2), a calcium-binding protein, is further cleaved into NES1 (Nesfatin-1). NES1 is an anorexigenic peptide and seems to participate in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. GADD45A (Growth arrest and DNA damage-inducible protein GADD45 alpha) has been identified as an interactor of Nucb2. Besides, rat GADD45A shares similarities with mouse NES1 in amino acid sequence with the identity of 98%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse NES1 and recombinant rat GADD45A. Briefly, NES1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL NES1 were then transferred to GADD45A-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NES1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of NES1 and GADD45A was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of NES1 with GADD45A.

Application Details

Application Notes: Isoelectric Point: 5.8

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: NUCB2 (NES1) (Nesfatin-1 (NES1))

Alternative Name: Nesfatin 1 (NES1) (NES1 Products)

Synonyms: nucleobindin-2 Protein, nucleobindin 2 Protein, NUCB2 Protein, Nucb2 Protein

Molecular Weight: 39kDa

UniProt: Q9JI85