IL-7 Protein (AA 26-177) (His tag)

Catalog No. ABIN7278663

This Recombinant IL-7 protein is expressed in HEK-293 Cells.

Quick Overview for IL-7 Protein (AA 26-177) (His tag) (ABIN7278663)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 26-177

Purification tag / Conjugate: This IL-7 protein is labelled with His tag.

Purpose: IL-7 (human) (rec.) (His)

Specificity: The extracellular domain of human IL-7 (aa 26-177) is fused at the C-terminus to a His-tag.

Characteristics: Protein. The extracellular domain of human IL-7 (aa 26-177) is fused at the C-terminus to a His-tag. Source: HEK 293 cells. Endotoxin content: <0.01EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >95 % (SDS-PAGE). Interleukin-7 (IL-7), is a hematopoietic growth factor and a member of the IL-7/IL-9 family. IL-7 stimulates the proliferation of lymphoid progenitors and is secreted by stromal cells in the bone marrow and thymus. IL-7 is also important for proliferation during certain stages of B cell maturation. IL-7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRbeta) during early T cell development. IL-7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes.

Endotoxin Level: <0.01EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Measured by the dose dependent stimulation of the proliferation of murine 2E8 cells. The ED50 for this effect is typically 0.2ng/ml corresponding to a specific activity of 5x 106 units/mg

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: IL-7

Background:

Alternate Names/Synonyms: Interleukin-7, Lymphopoietin 1, LP-1

Product Description: Interleukin-7 (IL-7), is a hematopoietic growth factor and a member of the IL-7/IL-9 family. IL-7 stimulates the proliferation of lymphoid progenitors and is secreted by stromal cells in the bone marrow and thymus. IL-7 is also important for proliferation during certain stages of B cell maturation. IL-7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRbeta) during early T cell development. IL-7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes.

Molecular Weight: ~27kDa (SDS-PAGE)

Pathways: JAK-STAT Signaling