IL-7 Protein (AA 26-177) (Fc Tag)

Catalog No. ABIN6253247

Recombinant IL-7 protein expressed in CHO Cells.

Quick Overview for IL-7 Protein (AA 26-177) (Fc Tag) (ABIN6253247)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Purity: >98 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 26-177

Purification tag / Conjugate: This IL-7 protein is labelled with Fc Tag.

Purpose: IL-7 (human):Fc (human) (rec.) (non-lytic)

Specificity: The extracellular domain of human IL-7 (aa 26-177) is fused to the N-terminus of the Fc portion of a mutant human IgG1.

Characteristics: Protein. The extracellular domain of human IL-7 (aa 26-177) is fused to the N-terminus of the Fc portion of a mutant human IgG1. Source: CHO cells. Endotoxin content: <0.06EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >98 % (SDS-PAGE). Interleukin-7 (IL-7), is a hematopoietic growth factor and a member of the IL-7/IL-9 family. IL-7 stimulates the proliferation of lymphoid progenitors and is secreted by stromal cells in the bone marrow and thymus. IL-7 is also important for proliferation during certain stages of B cell maturation. IL-7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRbeta) during early T cell development. IL-7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes.

Endotoxin Level: <0.06EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Measured in a cell proliferation assay using antibody against CD3-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is typically 10-100ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: IL-7

Background:

Alternate Names/Synonyms: Interleukin-7

Product Description: Interleukin-7 (IL-7), is a hematopoietic growth factor and a member of the IL-7/IL-9 family. IL-7 stimulates the proliferation of lymphoid progenitors and is secreted by stromal cells in the bone marrow and thymus. IL-7 is also important for proliferation during certain stages of B cell maturation. IL-7 and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. It is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRbeta) during early T cell development. IL-7 can be produced locally by intestinal epithelial and epithelial goblet cells and may serve as a regulatory factor for intestinal mucosal lymphocytes.

Pathways: JAK-STAT Signaling