IL12 Protein (AA 23-328, AA 57-253) (Fc Tag)

Catalog No. ABIN6253329

This Recombinant IL12 protein is produced in CHO Cells.

Quick Overview for IL12 Protein (AA 23-328, AA 57-253) (Fc Tag) (ABIN6253329)

Target: IL12 (Interleukin 12 (IL12))

Protein Type: Recombinant

Origin: Human

Source: CHO Cells

Purity: >98 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 23-328, AA 57-253

Purification tag / Conjugate: This IL12 protein is labelled with Fc Tag.

Purpose: IL-12 (human):Fc (human) (rec.)

Specificity: The human IL-12 complex composed of the p40 subunit (aa 23-328) and the p35 subunit (aa 57-253) is fused through a polypeptide linker to the Fc region of human IgG1.

Characteristics: Protein. The human IL-12 complex composed of the p40 subunit (aa 23-328) and the p35 subunit (aa 57-253) is fused through a polypeptide linker to the Fc region of human IgG1. Source: CHO cells. Endotoxin content: <0.06EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >98 % (SDS-PAGE). Interleukin-12 (IL-12), also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL-12 activities include the induction of IFN-gamma and TNF in resting and activated T and NK cells, the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen, and the enhancement of NK cell proliferation. Current evidence indicates that IL-12 is a key mediator of cellular immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular immunity and as an antitumor and anti viral agent.

Endotoxin Level: <0.06EU/μg protein (LAL test, Lonza).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12

Background:

Alternate Names/Synonyms: Interleukin-12, IL-12p70, IL-12p75, Cytotoxic Lymphocyte Maturation Factor, CLMF, NKSF, Natural Killer Cell Stimulatory Factor, TSF, T-cell Stimulatory Factor

Product Description: Interleukin-12 (IL-12), also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL-12 activities include the induction of IFN-gamma and TNF in resting and activated T and NK cells, the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen, and the enhancement of NK cell proliferation. Current evidence indicates that IL-12 is a key mediator of cellular immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular immunity and as an antitumor and anti viral agent.

NCBI Accession: NP_000873

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome