VTCN1 Protein (AA 29-258) (Fc Tag)

Catalog No. ABIN6253362

This Recombinant VTCN1 protein is produced in CHO Cells.

Quick Overview for VTCN1 Protein (AA 29-258) (Fc Tag) (ABIN6253362)

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Protein Type: Recombinant

Origin: Mouse

Source: CHO Cells

Purity: >98 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 29-258

Purification tag / Conjugate: This VTCN1 protein is labelled with Fc Tag.

Purpose: B7-H4 (mouse):Fc (mouse) (rec.)

Specificity: The extracellular domain of mouse B7-H4 (aa 29-258) is fused to the N-terminus of the Fc region of mouse IgG2a.

Characteristics: Protein. The extracellular domain of mouse B7-H4 (aa 29-258) is fused to the N-terminus of the Fc region of mouse IgG2a. Source: CHO cells. Endotoxin content: <0.06EU/μg protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >98 % (SDS-PAGE). B7-H4 is a B7 family member that negatively regulates T cell immunity by inhibiting T cell proliferation, cytokine production and cell cycle progression. In vitro, B7-H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production and generation of alloreactive cytotoxic T cells (CTLs). In vivo, blockade of endogenous B7-H4 by specific monoclonal antibody promotes T cell responses. B7-H4 ia an important negative regulator of innate immunity through growth inhibition of neutrophils. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance.

Endotoxin Level: <0.06EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Measured by its ablility to inhibit anti-CD3-induced proliferation of stimulated mouse T cells.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Alternative Name: B7-H4

Background:

Alternate Names/Synonyms: V-set Domain-containing T Cell Activation Inhibitor 1, VTCN1, B7h.5, Immune Costimulatory Protein B7-H4, T Cell Costimulatory Molecule B7x, Protein B7S1

Product Description: B7-H4 is a B7 family member that negatively regulates T cell immunity by inhibiting T cell proliferation, cytokine production and cell cycle progression. In vitro, B7-H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production and generation of alloreactive cytotoxic T cells (CTLs). In vivo, blockade of endogenous B7-H4 by specific monoclonal antibody promotes T cell responses. B7-H4 ia an important negative regulator of innate immunity through growth inhibition of neutrophils. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance.

NCBI Accession: NP_848709