Calreticulin Protein (CALR) (His tag)
Quick Overview for Calreticulin Protein (CALR) (His tag) (ABIN6121531)
Target
See all Calreticulin (CALR) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Calreticulin protein is labelled with His tag.
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Purpose
- Rat Calreticulin (CALR) Protein
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Sequence
- ARFYALSAR FEPFSNKGQT LVVQFTVKHE QNIDCGGGYV KLFPGGLDQK DMHGDSEYNI MFGPDICGPG TKKVHVIFNY KGKNVLINKD IRCKDDEFTH LYTLIVRPDN TYEVKIDNSQ VESGSLEDDW DFLPPKKIKD PDAAKPEDWD ERAKIDDPTD SKPEDWDKPE HIPDPDAKKP EDWDEEMDGE WEPPVIQNPE YKGEWKPRQI DNPDYKGTWI HPEIDNPEYS PDANIYAYDS FAVLGLDLWQ VK
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Biological Activity Comment
- Not tested
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Calreticulin (CALR) (AA 18-417), (C-Term) protein (His tag)
CALR Origin: Human Host: Escherichia coli (E. coli) Recombinant >90 % (SDS-PAGE) SDS
Calreticulin (CALR) (AA 18-417) protein (His tag)CALR Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Calreticulin (CALR) (AA 25-199) protein (His-IF2DI Tag)CALR Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Calreticulin (CALR) (AA 18-417) protein (His tag)CALR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
Calreticulin (CALR) (AA 1-417) protein (GST tag)CALR Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Please note that the majority of this manufacturers proteins are partial length rather than full length.
We recommend customers to inquire. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- Prior to lyophilization: PBS, pH 7.4, containing 0.01 % Sarcosyl, 5 % Trehalose.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze-thaw cycles.
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- Calreticulin (CALR)
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Alternative Name
- Calreticulin
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Molecular Weight
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Calculated MW: 32.7 kDa
Observed MW (SDS-PAGE): 38 kDa Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.Gene ID
- 64202
UniProt
- P18418
Pathways
- Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response
Target
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