BTN3A2 Protein (AA 30-248) (His tag,AVI tag,Biotin)

Catalog No. ABIN6386438

Product Details

Target: BTN3A2 (Butyrophilin, Subfamily 3, Member A2 (BTN3A2))

Protein Type: Recombinant

Protein Characteristics: AA 30-248

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This BTN3A2 protein is labelled with His tag,AVI tag,Biotin.

Sequence: AA 30-248

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: BTN3A2 (Butyrophilin, Subfamily 3, Member A2 (BTN3A2))

Alternative Name: BTN3A2 (BTN3A2 Products)

Synonyms: BT3.2 Protein, BTF4 Protein, CD277 Protein, butyrophilin subfamily 3 member A2 Protein, butyrophilin-like protein 1 Protein, butyrophilin subfamily 1 member A1 Protein, BTN3A2 Protein, LOC481727 Protein, LOC615223 Protein, LOC100599692 Protein

Background: Butyrophilin subfamily 3 member A2(BTN3A2/BT3.2) mRNA expression by tumoral cells was previously identified as a prognostic factor in a small cohort of high grade serous epithelial ovarian cancer (HG-EOC). We find that the three BTN3A isoforms: BTN3A1, BTN3A2, and BTN3A3, have high structural homology to the B7 superfamily of proteins and exist as V-shaped homodimers in solution, associating through the membrane proximal C-type Ig domain.

Molecular Weight: 27.3 kDa

NCBI Accession: NP_001184175