SYF2 Protein (AA 94-243) (His tag)

Catalog No. ABIN6386826

This Recombinant SYF2 protein is produced in Escherichia coli (E. coli).

Quick Overview for SYF2 Protein (AA 94-243) (His tag) (ABIN6386826)

Target: SYF2 (SYF2 Homolog, RNA Splicing Factor (SYF2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 94-243

Purification tag / Conjugate: This SYF2 protein is labelled with His tag.

Sequence: DYEKVKL LEISAEDAER WERKKKRKNP DLGFSDYAAA QLRQYHRLTK QIKPDMETYE RLREKHGEEF FPTSNSLLHG THVPSTEEID RMVIDLEKQI EKRDKYSRRR PYNDDADIDY INERNAKFNK KAERFYGKYT AEIKQNLERG TAV

Characteristics: SYF2, 94-243aa, Human, His tag, E.coli

Purification: > 90% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 50 % glycerol, 0.2M NaCl, 5 mM DTT, 2 mM EDTA

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: SYF2 (SYF2 Homolog, RNA Splicing Factor (SYF2))

Alternative Name: SYF2

Background: SYF2, also known as pre-mRNA-splicing factor SYF2, may be involved in pre-mRNA splicing. This protein interacts with cyclin D-type binding-protein 1, which is thought to be a cell cycle regulator at the G1/S transition. It is abundantly expressed in the heart, skeletal muscle and kidney and is expressed at lower levels in other tissues. Recombinant human SYF2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Molecular Weight: 20.5 kDa (173aa) confirmed by MALDI-TOF

NCBI Accession: NP_056299