TEF Protein (AA 1-303) (His tag)
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- Target See all TEF Proteins
- TEF (Thyrotrophic Embryonic Factor (TEF))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-303
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This TEF protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMSDAGGG KKPPVDPQAG PGPGPGRAAG ERGLSGSFPL VLKKLMENPP REARLDKEKG KEKLEEDEAA AASTMAVSAS LMPPIWDKTI PYDGESFHLE YMDLDEFLLE NGIPASPTHL AHNLLLPVAE LEGKESASSS TASPPSSSTA IFQPSETVSS TESSLEKERE TPSPIDPNCV EVDVNFNPDP ADLVLSSVPG GELFNPRKHK FAEEDLKPQP MIKKAKKVFV PDEQKDEKYW TRRKKNNVAA KRSRDARRLK ENQITIRAAF LEKENTALRT EVAELRKEVG KCKTIVSKYE TKYGPL
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product TEF Protein
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Thyrotrophic Embryonic Factor (TEF) (AA 1-303) protein (His tag)
TEF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Thyrotrophic Embryonic Factor (TEF) (AA 1-303) protein (GST tag)TEF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Thyrotrophic Embryonic Factor (TEF) (AA 214-303) protein (GST tag)TEF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Thyrotrophic Embryonic Factor (TEF) (AA 1-303) protein (Strep Tag)Crystallography grade TEF Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Thyrotrophic Embryonic Factor (TEF) (AA 1-301) protein (Strep Tag)Crystallography grade TEF Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- TEF (Thyrotrophic Embryonic Factor (TEF))
- Alternative Name
- TEF (TEF Products)
- Synonyms
- 2310028D20Rik Protein, tef Protein, tefb Protein, tefbeta Protein, wu:fa12f02 Protein, wu:fb11h02 Protein, zTEF[a] Protein, VBP Protein, xtef Protein, TEF Protein, DKFZp459A027 Protein, dbp Protein, TEF2 Protein, zgc:112180 Protein, TEF, PAR bZIP transcription factor Protein, thyrotroph embryonic factor Protein, thyrotrophic embryonic factor a Protein, thyrotrophic embryonic factor Protein, D site of albumin promoter (albumin D-box) binding protein L homeolog Protein, thyrotrophic embryonic factor b Protein, TEF Protein, Tef Protein, tefa Protein, tef Protein, dbp.L Protein, tefb Protein
- Background
- Thyrotroph embryonic factor isoform 1, also known as TEF, is a 303 amino acid nuclear transcription factor that belongs to the bZIP (basic region/leucine zipper) family and PAR (proline and acidic amino acid-rich) subfamily. TEF binds DNA as either a homodimer or heterodimer, and is known to transactivate the TSH beta promoter. TEF accumulates according to a robust circadian rhythm and has also been found to inhibit cell growth by down-regulating beta chain expression of cytokine receptors. Recombinant human TEF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 35.6kDa (326aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
- NCBI Accession
- NP_003207
- UniProt
- Q10587
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