RFXANK Protein (AA 1-237) (His tag)
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- Target See all RFXANK Proteins
- RFXANK (Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-237
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RFXANK protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMELTQPA EDLIQTQQTP ASELGDPEDP GEEAADGSDT VVLSLFPCTP EPVNPEPDAS VSSPQGSSLK HSTTLTNRQR GNEVSALPAT LDCDNLVNKP DERGFTPLIW ASAFGEIETV RFLLEWGADP HILAKERESA LSLASTGGYT DIVGLLLERD VDINIYDWNG GTPLLYAVRG NHVKCVEALL ARGADLTTEA DSGYTPMDLA VALGYRKVQQ VIENHILKLF QSNLVPADPE
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product RFXANK Protein
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Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK) (AA 1-237) protein (His tag)
RFXANK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK) (AA 1-237) protein (GST tag)RFXANK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK) (AA 1-90) protein (GST tag)RFXANK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK) (AA 1-260) protein (Strep Tag)Crystallography grade RFXANK Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK) (AA 1-269) protein (Strep Tag)Crystallography grade RFXANK Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)RFXANK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 20 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- RFXANK (Regulatory Factor X-Associated Ankyrin Containing Protein (RFXANK))
- Alternative Name
- RFXANK (RFXANK Products)
- Synonyms
- ANKRA1 Protein, BLS Protein, F14150_1 Protein, RFX-B Protein, Tvl1 Protein, regulatory factor X associated ankyrin containing protein Protein, regulatory factor X-associated ankyrin-containing protein Protein, RFXANK Protein, Rfxank Protein
- Background
- RFXANK, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Two transcript variants encoding different isoforms have been described for this gene, with only one isoform showing activation activity. Recombinant human RFXANK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 28 kDa (260aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_604389
- UniProt
- O14593
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