RDBP Protein (AA 1-380) (His tag)
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- Target See all RDBP Proteins
- RDBP (RD RNA Binding Protein (RDBP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-380
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RDBP protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMLVIPPG LSEEEEALQK KFNKLKKKKK ALLALKKQSS SSTTSQGGVK RSLSEQPVMD TATATEQAKQ LVKSGAISAI KAETKNSGFK RSRTLEGKLK DPEKGPVPTF QPFQRSISAD DDLQESSRRP QRKSLYESFV SSSDRLRELG PDGEEAEGPG AGDGPPRSFD WGYEERSGAH SSASPPRSRS RDRSHERNRD RDRDRERDRD RDRDRDRERD RDRDRDRDRD RERDRDRERD RDRDREGPFR RSDSFPERRA PRKGNTLYVY GEDMTPTLLR GAFSPFGNII DLSMDPPRNC AFVTYEKMES ADQAVAELNG TQVESVQLKV NIARKQPMLD AATGKSVWGS LAVQNSPKGC HRDKRTQIVY SDDVYKENLV DGF
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product RDBP Protein
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RD RNA Binding Protein (RDBP) (AA 1-380) protein (His tag)
RDBP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
RD RNA Binding Protein (RDBP) (AA 281-379) protein (GST tag)RDBP Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
RD RNA Binding Protein (RDBP) (AA 1-380) protein (GST tag)RDBP Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
RD RNA Binding Protein (RDBP) (AA 1-375) protein (Strep Tag)Crystallography grade RDBP Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 50 % glycerol, 5 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- RDBP (RD RNA Binding Protein (RDBP))
- Alternative Name
- NELFE (RDBP Products)
- Synonyms
- CG5994 Protein, Dmel\\CG5994 Protein, NELF Protein, NELF-E Protein, Nelf-e Protein, anon-66Da Protein, cg5994 Protein, dNelf-E Protein, D6S45 Protein, RD Protein, RDBP Protein, RDP Protein, Rd Protein, Rdbp Protein, D17H6S45 Protein, Negative elongation factor E Protein, negative elongation factor complex member E Protein, negative elongation factor complex member E, Rdbp Protein, Nelf-E Protein, NELFE Protein, Nelfe Protein
- Background
- NELFE is a part of complex termed negative elongation factor (NELF) which represses RNA polymerase II transcript elongation. This protein bears similarity to nuclear RNA-binding proteins, however, it has not been demonstrated that this protein binds RNA. It contains a tract of alternating basic and acidic residues, largely arginine (R) and aspartic acid (D). Recombinant human NELFE protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
- Molecular Weight
- 45.6kDa (403aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_002895
- UniProt
- P18615
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