PREP Protein (AA 1-710) (His tag)
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- Target See all PREP Proteins
- PREP (Prolyl Endopeptidase (PREP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-710
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PREP protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMLSLQYP DVYRDETAVQ DYHGHKICDP YAWLEDPDSE QTKAFVEAQN KITVPFLEQC PIRGLYKERM TELYDYPKYS CHFKKGKRYF YFYNTGLQNQ RVLYVQDSLE GEARVFLDPN ILSDDGTVAL RGYAFSEDGE YFAYGLSASG SDWVTIKFMK VDGAKELPDV LERVKFSCMA WTHDGKGMFY NSYPQQDGKS DGTETSTNLH QKLYYHVLGT DQSEDILCAE FPDEPKWMGG AELSDDGRYV LLSIREGCDP VNRLWYCDLQ QESSGIAGIL KWVKLIDNFE GEYDYVTNEG TVFTFKTNRQ SPNYRVINID FRDPEESKWK VLVPEHEKDV LEWIACVRSN FLVLCYLHDV KNILQLHDLT TGALLKTFPL DVGSIVGYSG QKKDTEIFYQ FTSFLSPGII YHCDLTKEEL EPRVFREVTV KGIDASDYQT VQIFYPSKDG TKIPMFIVHK KGIKLDGSHP AFLYGYGGFN ISITPNYSVS RLIFVRHMGG ILAVANIRGG GEYGETWHKG GILANKQNCF DDFQCAAEYL IKEGYTSPKR LTINGGSNGG LLVAACANQR PDLFGCVIAQ VGVMDMLKFH KYTIGHAWTT DYGCSDSKQH FEWLVKYSPL HNVKLPEADD IQYPSMLLLT ADHDDRVVPL HSLKFIATLQ YIVGRSRKQS NPLLIHVDTK AGHGAGKPTA KVIEEVSDMF AFIARCLNVD WIP
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product PREP Protein
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Prolyl Endopeptidase (PREP) (AA 1-710) protein (GST tag)
PREP Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Prolyl Endopeptidase (PREP) (AA 611-710) protein (GST tag)PREP Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Prolyl Endopeptidase (PREP) protein (His tag)PREP Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE
Prolyl Endopeptidase (PREP) (AA 1-710) protein (Strep Tag)Crystallography grade PREP Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Prolyl Endopeptidase (PREP) (AA 1-710) protein (Strep Tag)Crystallography grade PREP Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Prolyl Endopeptidase (PREP) protein (Myc-DYKDDDDK Tag)PREP Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In PBS buffer ( pH 7.4) containing 30 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PREP (Prolyl Endopeptidase (PREP))
- Alternative Name
- PREP (PREP Products)
- Synonyms
- PE Protein, PEP Protein, AI047692 Protein, AI450383 Protein, D10Wsu136e Protein, Pop Protein, rPop Protein, im:7140031 Protein, zgc:110670 Protein, prolyl endopeptidase Protein, Prolyl endopeptidase Protein, RB12337 Protein, AM1_4884 Protein, ppce Protein, PREP Protein, Prep Protein, prep Protein
- Background
- PREP is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides. Recombinant human PREP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 83.1kDa (733aa)
- NCBI Accession
- NP_002717
- UniProt
- P48147
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