NAGA Protein (AA 18-411) (His tag)
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- Target See all NAGA Proteins
- NAGA (N-Acetylgalactosaminidase, alpha (NAGA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 18-411
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This NAGA protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- LDNGLLQTPP MGWLAWERFR CNINCDEDPK NCISEQLFME MADRMAQDGW RDMGYTYLNI DDCWIGGRDA SGRLMPDPKR FPHGIPFLAD YVHSLGLKLG IYADMGNFTC MGYPGTTLDK VVQDAQTFAE WKVDMLKLDG CFSTPEERAQ GYPKMAAALN ATGRPIAFSC SWPAYEGGLP PRVNYSLLAD ICNLWRNYDD IQDSWWSVLS ILNWFVEHQD ILQPVAGPGH WNDPDMLLIG NFGLSLEQSR AQMALWTVLA APLLMSTDLR TISAQNMDIL QNPLMIKINQ DPLGIQGRRI HKEKSLIEVY MRPLSNKASA LVFFSCRTDM PYRYHSSLGQ LNFTGSVIYE AQDVYSGDII SGLRDETNFT VIINPSGVVM WYLYPIKNLE MSQQHHHHHH
- Purity
- > 90 % by SDS - PAGE.
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product NAGA Protein
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N-Acetylgalactosaminidase, alpha (NAGA) (AA 210-321) protein (His-IF2DI Tag)
NAGA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
N-Acetylgalactosaminidase, alpha (NAGA) (AA 1-411) protein (GST tag)NAGA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
N-Acetylgalactosaminidase, alpha (NAGA) (AA 18-411) protein (His tag)Crystallography grade NAGA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N-Acetylgalactosaminidase, alpha (NAGA) (AA 18-415) protein (His tag)Crystallography grade NAGA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- NAGA (N-Acetylgalactosaminidase, alpha (NAGA))
- Alternative Name
- NAGA (NAGA Products)
- Synonyms
- D22S674 Protein, GALB Protein, alpha-N-acetylgalactosaminidase Protein, N-acetyl galactosaminidase, alpha Protein, Naga Protein, NAGA Protein
- Background
- NAGA, also known as alpha-N-acetylgalactosaminidase, is a lysosomal exoglycosidase that cleaves terminal alpha-N-acetylgalactosamine residues from glycopeptides and glycolipids. It is hardly expected to cause an allergic reaction in Fabry disease patients. It is highly promising as a new and safe enzyme for ERT for Fabry disease. Recombinant human NAGA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 45.5kDa (400aa) 40-57kDa (SDS-PAGE under reducing conditions
- NCBI Accession
- NP_000253
- UniProt
- P17050
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