GUK1 Protein (AA 1-197) (His tag)

Catalog No. ABIN6387883

Product Details

Target: GUK1 (Guanylate Kinase 1 (GUK1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-197

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This GUK1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE

Purity: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 90 units/mg, and is defined as the amount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl buffer (  pH 8.0) containing 1 mM DTT, 1 mM EDTA, 20 % glycerol

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: GUK1 (Guanylate Kinase 1 (GUK1))

Alternative Name: GUK1 (GUK1 Products)

Synonyms: GMK Protein, AA409738 Protein, AL033299 Protein, AL033300 Protein, AV026611 Protein, Gmk Protein, Guk-1 Protein, guk1 Protein, zgc:73128 Protein, zgc:73217 Protein, gmk Protein, GUK1 Protein, AGK1 Protein, T11A7.23 Protein, guanylate kinase 1 Protein, DDBDRAFT_0215291 Protein, DDBDRAFT_0230100 Protein, DDB_0215291 Protein, DDB_0230100 Protein, sb:cb295 Protein, zgc:86776 Protein, guanylate kinase 1 Protein, guanylate kinase (GUK1) Protein, guanylate kinase Protein, guanylate kinase 1b Protein, guanylate kinase 1 L homeolog Protein, guanylate kinase 1a Protein, GUK1 Protein, Guk1 Protein, guk1b Protein, guk1 Protein, guk1.L Protein, GK-1 Protein, gmk Protein, gmkA Protein, Mrub_2093 Protein, Arnit_0783 Protein, Ndas_3097 Protein, Mesil_1319 Protein, Slip_0854 Protein, Olsu_0986 Protein, Acear_1451 Protein, AOR_1_2886174 Protein, guk1a Protein

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer composed of 36-  kDa subunits and has various intracellular functions. GAPDH catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Besides functioning as a glycolytic enzyme in cytoplasm, evidence suggests that mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recombinant GAPDH protein was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 36kDa (335aa), confirmed by MALDI-TOF

NCBI Accession: NP_002037

UniProt: P04406

Pathways: Nucleotide Phosphorylation, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process