Glucuronidase beta Protein (AA 23-651) (His tag)

Catalog No. ABIN7529348

Product Details

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 23-651

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This Glucuronidase beta protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: LQGGMLYPQE SPSRECKELD GLWSFRADFS DNRRRGFEEQ WYRRPLWESG PTVDMPVPSS FNDISQDWRL RHFVGWVWYE REVILPERWT QDLRTRVVLR IGSAHSYAIV WVNGVDTLEH EGGYLPFEAD ISNLVQVGPL PSRLRITIAI NNTLTPTTLP PGTIQYLTDT SKYPKGYFVQ NTYFDFFNYA GLQRSVLLYT TPTTYIDDIT VTTSVEQDSG LVNYQISVKG SNLFKLEVRL LDAENKVVAN GTGTQGQLKV PGVSLWWPYL MHERPAYLYS LEVQLTAQTS LGPVSDFYTL PVGIRTVAVT KSQFLINGKP FYFHGVNKHE DADIRGKGFD WPLLVKDFNL LRWLGANAFR TSHYPYAEEV MQMCDRYGIV VIDECPGVGL ALPQFFNNVS LHHHMQVMEE VVRRDKNHPA VVMWSVANEP ASHLESAGYY LKMVIAHTKS LDPSRPVTFV SNSNYAADKG APYVDVICLN SYYSWYHDYG HLELIQLQLA TQFENWYKKY QKPIIQSEYG AETIAGFHQD PPLMFTEEYQ KSLLEQYHLG LDQKRRKYVV GELIWNFADF MTEQSPTRVL GNKKGIFTRQ RQPKSAAFLL RERYWKIANE TRYPHSVAKS QCLENSLFTH HHHHH

Purity: > 90 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 1600 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-Methylumbelliferone to 4-Methylum-belliferyl-beta-D-glucosiduronic acid per minute at 37C and pH6.0

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Alternative Name: GUSB (GUSB Products)

Synonyms: GUSB Protein, beta-GUS Protein, gus Protein, BG Protein, MPS7 Protein, AI747421 Protein, Gur Protein, Gus Protein, Gus-r Protein, Gus-s Protein, Gus-t Protein, Gus-u Protein, Gut Protein, asd Protein, g Protein, Ac2-223 Protein, si:ch211-160e1.7 Protein, si:ct573103.7 Protein, glucuronidase beta Protein, beta-D-glucuronidase Protein, beta-glucuronidase Protein, beta glucuronidase Protein, glucuronidase, beta Protein, GUSB Protein, bglR Protein, SSO_RS14735 Protein, Glu Protein, Gusb Protein, gusb Protein

Background: GUSB, also known as beta-glucuronidase isoform 1, is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It includes heparin sulfate, chondroitin sulfate and hyaluronan. Mutations in the GUSB are linked to mucopolysaccharidosis type VII. GUSB plays an important role in the degradation of dermatan and keratin sulfates. Recombinant human GUSB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 73.4kDa (635aa) 70-100KDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_000172

UniProt: P08236

Pathways: Glycosaminoglycan Metabolic Process