IMPAD1 Protein (AA 34-356) (His tag)

Catalog No. ABIN6387946

Product Details

Target: IMPAD1 (Inositol Monophosphatase Domain Containing 1 (IMPAD1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 34-356

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This IMPAD1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: ADPGRFSLFG LGSEPAAGEA EVASDGGTVD LREMLAVAVL AAERGGDEVR RVRESNVLHE KSKGKTREGA DDKMTSGDVL SNRKMFYLLK TAFPNVQINT EEHVDASDKE VIVWNRKIPE DILKEIAAPK EVPAESVTVW IDPLDATQEY TEDLRKYVTT MVCVAVNGKP VLGVIHKPFS EYTAWAMVDG GSNVKARSSY NEKTPKIIVS RSHAGMVKQV ALQTFGNQTS IIPAGGAGYK VLALLDVPDM TQEKADLYIH VTYIKKWDIC AGNAILKALG GHMTTLNGEE ISYTGSDGIE GGLLASIRMN HQALVRKLPD LEKSGHHHHH HH

Purity: > 95 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 5,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze Adenosine 3, 5-diphosphate per minute at pH 7.5 at 25C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: IMPAD1 (Inositol Monophosphatase Domain Containing 1 (IMPAD1))

Alternative Name: Impad1 (IMPAD1 Products)

Synonyms: IMP 3 Protein, RGD1306455 Protein, gPAPP Protein, impa3 Protein, 1110001C20Rik Protein, AA408880 Protein, AI451589 Protein, AL022796 Protein, B230207P20 Protein, Jaws Protein, GPAPP Protein, IMP-3 Protein, IMPA3 Protein, IMPase 3 Protein, zgc:123256 Protein, inositol monophosphatase domain containing 1 Protein, inositol monophosphatase domain containing 1 S homeolog Protein, Impad1 Protein, impad1.S Protein, IMPAD1 Protein, impad1 Protein

Background: IMPAD1, also known as inositol monophosphatase 3, may play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3, 5-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. Recombinant mouse IMPAD1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 36.2kDa (332aa) 28-40KDa (SDS-PAGE under non-reducing conditions.)

NCBI Accession: NP_808398

UniProt: Q80V26

Pathways: Glycosaminoglycan Metabolic Process