DUSP18 Protein (AA 1-188) (His tag)
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- Target See all DUSP18 Proteins
- DUSP18 (Dual Specificity Phosphatase 18 (DUSP18))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-188
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This DUSP18 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSHMTAPSC AFPVQFRQPS VSGLSQITKS LYISNGVAAN NKLMLSSNQI TMVINVSVEV VNTLYEDIQY MQVPVADSPN SRLCDFFDPI ADHIHSVEMK QGRTLLHCAA GVSRSAALCL AYLMKYHAMS LLDAHTWTKS CRPIIRPNSG FWEQLIHYEF QLFGKNTVHM VSSPVGMIPD IYEKEVRLMI PL
- Purity
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 300 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C
- Top Product
- Discover our top product DUSP18 Protein
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Dual Specificity Phosphatase 18 (DUSP18) (AA 1-188) protein (His tag)
DUSP18 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Dual Specificity Phosphatase 18 (DUSP18) (AA 1-188) protein (GST tag)DUSP18 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Dual Specificity Phosphatase 18 (DUSP18) (AA 1-188) protein (Strep Tag)Crystallography grade DUSP18 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Dual Specificity Phosphatase 18 (DUSP18) (AA 1-188) protein (Strep Tag)Crystallography grade DUSP18 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Dual Specificity Phosphatase 18 (DUSP18) protein (Myc-DYKDDDDK Tag)DUSP18 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 40 % glycerol, 0.1 mM PMSF, 1 mM EDTA
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- DUSP18 (Dual Specificity Phosphatase 18 (DUSP18))
- Alternative Name
- DUSP18 (DUSP18 Products)
- Synonyms
- MGC131167 Protein, DSP18 Protein, DUSP20 Protein, LMWDSP20 Protein, bK963H5.1 Protein, 4930527G07Rik Protein, AI430870 Protein, BB028064 Protein, dual specificity phosphatase 18 Protein, dual specificity phosphatase 18 L homeolog Protein, DUSP18 Protein, dusp18.L Protein, Dusp18 Protein
- Background
- Dual specificity phosphatase 18, also known as DUSP18, is a member of the dual-specificity phosphatase (DSP) family, which catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues. DUSP18 is inhibited by iodoarectic acid and is activated by manganese ions. Along with having preferential enzymatic activity against phosphorylated tyrosine residues over threonine residues, DUSP18 also dephosphorylates p-nitrophenyl phosphate (pNPP) in vitro. This protein is widely expressed with highest levels in liver, brain, ovary and testis. Recombinant human DUSP18 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 23.6 kDa (212aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_689724
- UniProt
- Q8NEJ0
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