PGP Protein (AA 1-321) (His tag)
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- Target See all PGP Proteins
- PGP (Phosphoglycolate Phosphatase (PGP))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-321
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PGP protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSHMAAAEA GGDDARCVRL SAERAQALLA DVDTLLFDCD GVLWRGETAV PGAPEALRAL RARGKRLGFI TNNSSKTRAA YAEKLRRLGF GGPAGPGASL EVFGTAYCTA LYLRQRLAGA PAPKAYVLGS PALAAELEAV GVASVGVGPE PLQGEGPGDW LHAPLEPDVR AVVVGFDPHF SYMKLTKALR YLQQPGCLLV GTNMDNRLPL ENGRFIAGTG CLVRAVEMAA QRQADIIGKP SRFIFDCVSQ EYGINPERTV MVGDRLDTDI LLGATCGLKT ILTLTGVSTL GDVKNNQESD CVSKKKMVPD FYVDSIADLL PALQG
- Purity
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 3,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
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- Discover our top product PGP Protein
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Phosphoglycolate Phosphatase (PGP) (AA 1-321) protein (His tag)
PGP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Phosphoglycolate Phosphatase (PGP) (AA 1-321) protein (Strep Tag)Crystallography grade PGP Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Phosphoglycolate Phosphatase (PGP) (AA 1-321) protein (Strep Tag)Crystallography grade PGP Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Phosphoglycolate Phosphatase (PGP) protein (Myc-DYKDDDDK Tag)PGP Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PGP (Phosphoglycolate Phosphatase (PGP))
- Alternative Name
- PGP (PGP Products)
- Synonyms
- RGD1307773 Protein, cb241 Protein, sb:cb241 Protein, wu:fb92c03 Protein, wu:fi40b02 Protein, zgc:56011 Protein, 1700012G19Rik Protein, AI481330 Protein, phosphoglycolate phosphatase Protein, Pgp Protein, PGP Protein, pgp Protein
- Background
- Phosphoglycolate phosphatase, also known as PGP, is detected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). The catalytic activity of PGP is 2-phosphoglycolate + H2O = glycolate + phosphate. The highest activities occur in skeletal muscle and cardiac muscle. Recombinant human PGP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 36.5 kDa (345aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_001035830
- UniProt
- A6NDG6
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