UMPS Protein (AA 1-480) (His tag)
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- Target See all UMPS Proteins
- UMPS (Uridine Monophosphate Synthetase (UMPS))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-480
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This UMPS protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MAVARAALGP LVTGLYDVQA FKFGDFVLKS GLSSPIYIDL RGIVSRPRLL SQVADILFQT AQNAGISFDT VCGVPYTALP LATVICSTNQ IPMLIRRKET KDYGTKRLVE GTINPGETCL IIEDVVTSGS SVLETVEVLQ KEGLKVTDAI VLLDREQGGK DKLQAHGIRL HSVCTLSKML EILEQQKKVD AETVGRVKRF IQENVFVAAN HNGSPLSIKE APKELSFGAR AELPRIHPVA SKLLRLMQKK ETNLCLSADV SLARELLQLA DALGPSICML KTHVDILNDF TLDVMKELIT LAKCHEFLIF EDRKFADIGN TVKKQYEGGI FKIASWADLV NAHVVPGSGV VKGLQEVGLP LHRGCLLIAE MSSTGSLATG DYTRAAVRMA EEHSEFVVGF ISGSRVSMKP EFLHLTPGVQ LEAGGDNLGQ QYNSPQEVIG KRGSDIIIVG RGIISAADRL EAAEMYRKAA WEAYLSRLGV HHHHHH
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Top Product
- Discover our top product UMPS Protein
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Uridine Monophosphate Synthetase (UMPS) (AA 1-480) protein (GST tag)
UMPS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Uridine Monophosphate Synthetase (UMPS) (AA 381-479) protein (GST tag)UMPS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Uridine Monophosphate Synthetase (UMPS) (AA 2-480) protein (His tag)Crystallography grade UMPS Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Uridine Monophosphate Synthetase (UMPS) (Active) protein (Myc-DYKDDDDK Tag)UMPS Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4).
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- UMPS (Uridine Monophosphate Synthetase (UMPS))
- Alternative Name
- UMPS (UMPS Products)
- Synonyms
- zgc:55702 Protein, zgc:55723 Protein, UMPS Protein, OPRT Protein, 1700095D23Rik Protein, AA408257 Protein, AL033308 Protein, BB164745 Protein, Orotidine 5'-phosphate decarboxylase Protein, uridine monophosphate synthetase Protein, uridine monophosphate synthetase L homeolog Protein, uridine 5'-monophosphate synthase Protein, umps-1 Protein, umps Protein, umps.L Protein, UMPS Protein, LOC100556619 Protein, Umps Protein
- Background
- UMPS, also known as uridine 5'-monophosphate synthase, is a bifunctional enzyme catalyzing the last two steps of de novo pyrimidine biosynthesis. It is an indispensable component in this metabolic pathway and is a target for antimalarial and antitumor drugs. Its expression in colorectal carcinoma tissues is not correlated with the toxicities of 5-FU-based regimen, but It in the normal tissues can help predict the toxicities associated with 5-FU. Recombinant human UMPS, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 53kDa (486aa) 50-70kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_000364
- UniProt
- P11172
- Pathways
- Ribonucleoside Biosynthetic Process
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