ADK Protein (AA 1-361) (His tag)

Catalog No. ABIN6388126

Product Details

Target: ADK (Adenosine Kinase (ADK))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-361

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ADK protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAADEP KPKKLKVEAP QALSENVLFG MGNPLLDISA VVDKDFLDKY SLKPNDQILA EDKHKELFDE LVKKFKVEYH AGGSTQNSMK VAQWLIQEPH KAATFFGCIG IDKFGEILKR KAADAHVDAH YYEQNEQPTG TCAACITGGN RSLVANLAAA NCYKKEKHLD LERNWVLVEK ARVYYIAGFF LTVSPESVLK VARYAAENNR VFTLNLSAPF ISQFFKEALM DVMPYVDILF GNETEAATFA REQGFETKDI KEIAKKAQAL PKVNSKRQRT VIFTQGRDDT IVAAENDVTA FPVLDQNQEE IIDTNGAGDA FVGGFLSQLV SDKPLTECIR AGHYAASVII RRTGCTFPEK PDFH

Purity: > 95 % by SDS - PAGE

Endotoxin Level: < 1.0 EU per 1 ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 100 pmol/min/ and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Bioactivity Validated

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-Hcl buffer (  pH 8.0) containing 20 % glycerol, 50  mM NaCl, 1  mM EDTA

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Target Details

Target: ADK (Adenosine Kinase (ADK))

Alternative Name: Adk (ADK Products)

Synonyms: AK Protein, ADK Protein, DDBDRAFT_0186795 Protein, DDBDRAFT_0230174 Protein, DDB_0186795 Protein, DDB_0230174 Protein, ATPADK1 Protein, F3G5.4 Protein, F3G5_4 Protein, adenosine kinase Protein, adk Protein, 2310026J05Rik Protein, 5033405D03Rik Protein, AI255373 Protein, AI987814 Protein, Ak Protein, adenosine kinase Protein, ATP:adenosine 5'-phosphotransferase Protein, Adenosine kinase Protein, adenosine kinase S homeolog Protein, ADK Protein, adk Protein, Adk Protein, CNN02130 Protein, LOC5572668 Protein, CMU_026370 Protein, adk.S Protein

Background: Adk, also known as Adenosine kinase, is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant mouse Adk, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 42.5 kDa (384aa) confirmed by MALDI-TOF

NCBI Accession: NP_598840

UniProt: P55264

Pathways: Ribonucleoside Biosynthetic Process