Adenylate Kinase 1 Protein (AK1) (AA 1-210) (His tag)
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- Target See all Adenylate Kinase 1 (AK1) Proteins
- Adenylate Kinase 1 (AK1)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-210
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Adenylate Kinase 1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMGCCVSS EPQEEGGRKT GEKLKKAKII FVVGGPGSGK GTQCEKIVQK YGYTHLSTGD LLRAEVSSGS ERGKKLSAIM EKGELVPLDT VLDMLRDAML AKVDSSNGFL IDGYPREVKQ GEEFEQKIGQ PTLLLYVDAG AETMTQRLLK RGETSGRVDD NEETIKKRLE TYYNATEPVI SFYDKRGIVR KVNAEGTVDT VFSEVCTYLD SLK
- Purity
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 150 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.
- Top Product
- Discover our top product AK1 Protein
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Adenylate Kinase 1 (AK1) (AA 1-210) protein (His tag)
AK1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Adenylate Kinase 1 (AK1) (AA 1-194) protein (GST tag)AK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Adenylate Kinase 1 (AK1) protein (His tag)AK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % as determined by reducing SDS-PAGE.
Adenylate Kinase 1 (AK1) (AA 1-194), (full length) protein (His-SUMO Tag)AK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Adenylate Kinase 1 (AK1) protein (Myc-DYKDDDDK Tag)AK1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In 20 mM Tris-Hcl buffer ( pH 8.0) containing 10 % glycerol, 1 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- Adenylate Kinase 1 (AK1)
- Alternative Name
- Ak1 (AK1 Products)
- Synonyms
- ADK-1 Protein, AK1 Protein, CG17146 Protein, DAK1 Protein, Dak1 Protein, Dmel\\CG17146 Protein, adk1 Protein, bs34e10.y1 Protein, ak5 Protein, ADENYLATE KINASE 1 Protein, MLE2.3 Protein, MLE2_3 Protein, adenylate kinase 1 Protein, Ak-1 Protein, B430205N08Rik Protein, Ak 1 Protein, zgc:91930 Protein, Myokinase Protein, Adenylate kinase 1 Protein, adenylate kinase 1 Protein, Adk1 Protein, ak1 Protein, AK1 Protein, ADK1 Protein, Ak1 Protein
- Background
- Ak1, also known as Adenylate kinase isoenzyme 1 isoform 1, is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes.It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in Ak1 are the cause of a form of hemolytic anemia. Recombinant mouse Ak1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 25.5 kDa (233aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_067490
- UniProt
- Q9R0Y5
- Pathways
- Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
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