nectin-3 Protein (AA 58-404) (His tag)
Quick Overview for nectin-3 Protein (AA 58-404) (His tag) (ABIN6388148)
Target
See all nectin-3 (NECTIN3) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 58-404
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Purification tag / Conjugate
- This nectin-3 protein is labelled with His tag.
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Sequence
- GPIIVEPHVT AVWGKNVSLK CLIEVNETIT QISWEKIHGK SSQTVAVHHP QYGFSVQGEY QGRVLFKNYS LNDATITLHN IGFSDSGKYI CKAVTFPLGN AQSSTTVTVL VEPTVSLIKG PDSLIDGGNE TVAAICIAAT GKPVAHIDWE GDLGEMESTT TSFPNETATI ISQYKLFPTR FARGRRITCV VKHPALEKDI RYSFILDIQY APEVSVTGYD GNWFVGRKGV NLKCNADANP PPFKSVWSRL DGQWPDGLLA SDNTLHFVHP LTFNYSGVYI CKVTNSLGQR SDQKVIYISD PPTTTTLQPT IQWHPSTADI EDLATEPKKL PFPLSTLATI KDDTIATLEH HHHHH
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Purification
- > 90% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Nectin cell adhesion molecule 3 (NECTIN3) (AA 58-400) (Active) protein (His-Avi Tag)
NECTIN3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Nectin cell adhesion molecule 3 (NECTIN3) (AA 58-400) (Active) protein (Biotin,His-Avi Tag)NECTIN3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Nectin cell adhesion molecule 3 (NECTIN3) (AA 58-400) (Active) protein (Fc Tag)NECTIN3 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Nectin cell adhesion molecule 3 (NECTIN3) (AA 58-400) (Active) protein (Fc Tag,AVI tag,Biotin)NECTIN3 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Nectin cell adhesion molecule 3 (NECTIN3) (AA 1-366) protein (GST tag)NECTIN3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Nectin cell adhesion molecule 3 (NECTIN3) (AA 58-400) protein (His tag)NECTIN3 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 20 % glycerol, 1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))
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Alternative Name
- Nectin-3
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Background
- NECTIN3, also known as nectin-3 isoform 1, is a member of the nectin family. It is proposed to initiate cell-cell adhesion to promote cell attachment and to allow subsequent formation of JAM- and cadherin-based intercellular junctions. It has been shown to induce endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Subsequent Nectin-3 activity adds strength to the junction through trans-interaction with a variety of molecules. Recombinant human NECTIN3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
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Molecular Weight
- 39.1 kDa (355aa)
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NCBI Accession
- NP_056295
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Pathways
- Cell-Cell Junction Organization
Target
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