PCOLCE Protein (AA 26-449) (His tag)
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- Target See all PCOLCE Proteins
- PCOLCE (Procollagen C-Endopeptidase Enhancer (PCOLCE))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 26-449
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This PCOLCE protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MRDKEETLPL EDGWWGPGTR SAAREDDSIR PFKVETSDEE IHDLHQRIDK FRFTPPLEDS CFHYGFNSNY LKKVISYWRN EFDWKKQVEI LNRYPHFKTK IEGLDIHFIH VKPPQLPAGH TPKPLLMVHG WPGSFYEFYK IIPLLTDPKN HGLSDEHVFE VICPSIPGYG FSEASSKKGF NSVATARIFY KLMLRLGFQE FYIQGGDWGS LICTNMAQLV PSHVKGLHLN MALVLSNFST LTLLLGQRFG RFLGLTERDV ELLYPVKEKV FYSLMRESGY MHIQCTKPDT VGSALNDSPV GLAAYILEKF STWTNTEFRY LEDGGLERKF SLDDLLTNVM LYWTTGTIIS SQRFYKENLG QGWMTQKHER MKVYVPTGFS AFPFELLHTP EKWVRFKYPK LISYSYMVRG GHFAAFEEPE LLAQDIRKFL SVLERQHHHH HH
- Purity
- > 85 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Top Product
- Discover our top product PCOLCE Protein
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Procollagen C-Endopeptidase Enhancer (PCOLCE) (AA 1-449) protein (GST tag)
PCOLCE Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Procollagen C-Endopeptidase Enhancer (PCOLCE) (AA 26-449) protein (His tag)Crystallography grade PCOLCE Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Procollagen C-Endopeptidase Enhancer (PCOLCE) (AA 25-468) protein (His tag)Crystallography grade PCOLCE Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Procollagen C-Endopeptidase Enhancer (PCOLCE) protein (Myc-DYKDDDDK Tag)PCOLCE Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 50 % glycerol, 1 mM DTT, 0.1M NaCl
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PCOLCE (Procollagen C-Endopeptidase Enhancer (PCOLCE))
- Alternative Name
- PCOLCE (PCOLCE Products)
- Synonyms
- PCPE Protein, PCPE-1 Protein, PCPE1 Protein, Astt-2 Protein, Astt2 Protein, P14 Protein, procollagen C-endopeptidase enhancer Protein, procollagen C-endopeptidase enhancer L homeolog Protein, procollagen C-endopeptidase enhancer protein Protein, PCOLCE Protein, Pcolce Protein, pcolce.L Protein
- Background
- EPHX1, also known as epoxide hydrolase 1, is an important bioconversion enzyme that converts epoxides from degradation of aromatics into trans-dihydrodiols that can be conjugated and excreted in the body. The epoxide hydrolase acts both in the activation and decryption of the epoxide. This enzyme plays a role in regulating the extensive oxidation state of heterologous and lipid-derived substrates. Mutations in this gene cause preeclampsia, lack of epoxidase, or increased epoxide hydrolase activity. Recombinant human EPHX1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 51.5kDa (442aa) 50-70kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_000111
- UniProt
- P07099
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