NCR1 Protein (AA 22-255)

Catalog No. ABIN666806

This Recombinant NCR1 protein is produced in Escherichia coli (E. coli).

Quick Overview for NCR1 Protein (AA 22-255) (ABIN666806)

Target: NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 22-255

Sequence: MQQQTLPKPF IWAEPHFMVP KEKQVTICCQ GNYGAVEYQL HFEGSLFAVD RPKPPERINK VKFYIPDMNS RMAGQYSCIY RVGELWSEPS NLLDLVVTEM YDTPTLSVHP GPEVISGEEV TFYCRLDTAT SMFLLLKEGR SSHVQRGYGK VQAEFPLGPV TTAHRGTYRC FGSYNNHAWS FPSEPVKLLV TGDIENTSLA PEDPTFPADT WGTYLLTTET GLQKDHALWD HTAQN

Characteristics: NCR, NKp46 ( Extracellular Ig-like domain , 22-255aa, human), Recombinant, E.coli

Purification: > 95% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol, 1 mM EDTA

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))

Alternative Name: NKp46/NCR1

Background: A natural cytotoxicity receptor (NCR) NKp46 has been shown to represent a novel NK cell-specific molecule involved in human NK cell activation. The natural cytotoxicity receptors (NCRs) are a recently characterized family of Ig-like activation receptors that appear to be major triggering receptors in tumor cell recognition. The three known NCRs include NKp46 and NKp30, which are expressed on circulating NKcells, and NKp44, which is expressed only on activating NK cells. NKp46 has been implicated in NK cell-mediated lysis of several autologous tumor cells and pathogen-infected cell lines. NKp46 has two extracellular Ig-like domains followed by a ~40 residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail. The extracellular Ig-like domain of NKp46 ( 22-255aa) was overexpressed in E. coli, and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.

Molecular Weight: 26.6 kDa (235aa) confirmed by MALDI-TOF

NCBI Accession: NP_004820

Pathways: Regulation of Leukocyte Mediated Immunity