NCR1 Protein (AA 22-254) (His tag)

Catalog No. ABIN7275348

Product Details

Target: NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))

Protein Type: Recombinant

Protein Characteristics: AA 22-254

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This NCR1 protein is labelled with His tag.

Purpose: Human NKp46/NCR1/CD335 Protein

Sequence: Gln22-Asn254

Characteristics: Recombinant Human NKp46/NCR1/CD335 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln22-Asn254.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human NKp46, His Tag at 0.2μg/ml (100μl/Well). Dose response curve for Anti-NKp46 Ab., hFc Tag with the EC50 of 9ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: NCR1 (Natural Cytotoxicity Triggering Receptor 1 (NCR1))

Alternative Name: NKp46 (NCR1 Products)

Synonyms: CD335 Protein, LY94 Protein, NK-p46 Protein, NKP46 Protein, Ly94 Protein, NKp46 Protein, KILR-1 Protein, Nk-p46 Protein, Nkp46 Protein, natural cytotoxicity triggering receptor 1 Protein, NCR1 Protein, Ncr1 Protein

Background: NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR).These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells.

Molecular Weight: 27.5 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.

Pathways: Regulation of Leukocyte Mediated Immunity