TXN Protein (AA 1-105)

Catalog No. ABIN666852

This Recombinant TXN protein is expressed in Escherichia coli (E. coli).

Quick Overview for TXN Protein (AA 1-105) (ABIN666852)

Target: TXN (Thioredoxin (TXN))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity: > 90 %

Product Details

Protein Characteristics: AA 1-105

Sequence: MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV

Characteristics: Thioredoxin-1 Human, Recombinant, E.coli

Purification: > 90% by SDS-PAGE

Biological Activity Comment: Specific activity is >150 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4)

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: TXN (Thioredoxin (TXN))

Alternative Name: Thioredoxin-1

Background: Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human thioredoxin-1 was overexpressed in E. coli and purified by using conventional chromatography techniques

Molecular Weight: 11.7 kDa (105aa) confirmed by MALDI-TOF

NCBI Accession: NP_003320

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis