ARF1 Protein (AA 1-181) (His tag)

Catalog No. ABIN667162

Recombinant ARF1 protein expressed in Escherichia coli (E. coli).

Quick Overview for ARF1 Protein (AA 1-181) (His tag) (ABIN667162)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 % by SDS - PAGE

Product Details

Protein Characteristics: AA 1-181

Purification tag / Conjugate: This ARF1 protein is labelled with His tag.

Characteristics: ARF1, 1-181aa, Human, His tag, E.coli

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/ml (determined by Bradford assay)

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT

Storage: 4 °C

Target Details

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: ARF1

Background: ARF1, also known as ADP-ribosylation factor 1, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. It is essential and ubiquitous in eukaryotes, as this protein is involved in vesicular transport and functioning via phospholipase D activation. ARF1 plays a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids. Recombinant human ARF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: ADP-ribosylation factor 1, ADP ribosylation factor 1. NCBI no.: NP_001649

Molecular Weight: 22.8 kDa (201aa) confirmed by MALDI-TOF

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process