Pre-mRNA Branch Site Protein p14 (SF3B14) (AA 1-125) protein (His tag)

Catalog No. ABIN667356

Product Details

Target: Pre-mRNA Branch Site Protein p14 (SF3B14)

Protein Type: Recombinant

Protein Characteristics: AA 1-125

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: His tag

Application: SDS-PAGE (SDS)

Characteristics: SF3B14, 1-125aa, Human, His tag, E.coli

Purity: > 95 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol

Storage: 4 °C

Target Details

Target: Pre-mRNA Branch Site Protein p14 (SF3B14)

Alternative Name: SF3B14 (SF3B14 Products)

Synonyms: 6030419K15Rik Protein, AV001342 Protein, Sf3b14 Protein, zgc:86708 Protein, HSPC175 Protein, Ht006 Protein, P14 Protein, SAP14 Protein, SF3B14a Protein, splicing factor 3B, subunit 6 Protein, splicing factor 3b, subunit 6 Protein, splicing factor 3b subunit 6 S homeolog Protein, splicing factor 3b subunit 6 Protein, Sf3b6 Protein, sf3b6 Protein, sf3b6.S Protein, SF3B6 Protein

Background: SF3B14, also known as SAP14, is a 125 amino acid nuclear protein that is a component of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. Required for the splicing of pre-mRNA, SF3B14 enters the spliceosome and associates with the pre-mRNA branch site facilitating the interaction of snRNP with the branch sites of U2 and U12 of the 17S U2 and the 18S U11/U12 snRNP complex. Recombinant human SF3B14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Splicing factor 3B, 14 kDa subunit, CGI-110, HSPC175, Ht006, P14, SAP14, SF3B14a. NCBI no.: NP_057131

Molecular Weight: 16.7 kDa (145aa), confirmed by MALDI-TOF