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p300 Protein (GST tag)

EP300 Origin: Human Host: Insect cells (Sf9) Recombinant >80% WB
Rockland
Catalog No. ABIN6699785
Supplier Product No.: 009-001-s27s
  • Target See all p300 (EP300) Proteins
    p300 (EP300) (E1A Binding Protein P300 (EP300))
    Protein Type
    Recombinant
    Origin
    • 6
    • 1
    • 1
    Human
    Source
    • 5
    • 2
    • 1
    Insect cells (Sf9)
    Purification tag / Conjugate
    This p300 protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-s27s
    Supplier
    Rockland
    Purpose
    KAT3B (EP300) recombinant protein-GST fusion protein
    Purification
    Recombinant human KAT3B (EP300) (532-1153, contains the catalytic domain) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.
    Purity
    >80%
    Top Product
    Discover our top product EP300 Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: KAT3B Protein is suitable for use in Western Blot. Expect a band approximately ~120 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.1 μg/μL
    Buffer
    KAT3B Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    p300 (EP300) (E1A Binding Protein P300 (EP300))
    Alternative Name
    EP300 (EP300 Products)
    Background

    Synonyms: KAT3B, EP300, p300, Histone acetyltransferase p300

    Background: KAT3B (also known as EP300) encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein that functions as a histone acetyltransferase. KAT3B regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. KAT3B binding is a highly accurate process and can be used for identifying enhancers and their associated activities (1). KAT3B is also useful in the study of the role of tissue-specific enhancers in human biology and diseases on a genome wide scale. Defects in KAT3B gene are a cause of Rubinstein-Taybi syndrome that play a important role in epithelial cancer and act as a classic tumor suppressor gene (2). KAT3B Protein is ideal for investigators involved in Signaling Proteins, Acetyl/Methyltransferase Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, and PKA/PKC Pathway research.

    NCBI Accession
    NM_001429
    Pathways
    p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size
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