p300 Protein (GST tag)
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- Target See all p300 (EP300) Proteins
- p300 (EP300) (E1A Binding Protein P300 (EP300))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This p300 protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-s27s
- Supplier
- Rockland
- Purpose
- KAT3B (EP300) recombinant protein-GST fusion protein
- Purification
- Recombinant human KAT3B (EP300) (532-1153, contains the catalytic domain) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.
- Purity
- >80%
- Top Product
- Discover our top product EP300 Protein
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- Application Notes
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Western_Blot_Dilution: User Optimized
Application_Note: KAT3B Protein is suitable for use in Western Blot. Expect a band approximately ~120 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1 μg/μL
- Buffer
- KAT3B Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- p300 (EP300) (E1A Binding Protein P300 (EP300))
- Alternative Name
- EP300 (EP300 Products)
- Background
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Synonyms: KAT3B, EP300, p300, Histone acetyltransferase p300
Background: KAT3B (also known as EP300) encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein that functions as a histone acetyltransferase. KAT3B regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. KAT3B binding is a highly accurate process and can be used for identifying enhancers and their associated activities (1). KAT3B is also useful in the study of the role of tissue-specific enhancers in human biology and diseases on a genome wide scale. Defects in KAT3B gene are a cause of Rubinstein-Taybi syndrome that play a important role in epithelial cancer and act as a classic tumor suppressor gene (2). KAT3B Protein is ideal for investigators involved in Signaling Proteins, Acetyl/Methyltransferase Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, and PKA/PKC Pathway research.
- NCBI Accession
- NM_001429
- Pathways
- p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size
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