p300 Protein (GST tag)

Catalog No. ABIN6699785

Supplier Product No.: 009-001-s27s

Product Details

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Purification tag / Conjugate: This p300 protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-s27s

Supplier: Rockland

Purpose: KAT3B (EP300) recombinant protein-GST fusion protein

Purification: Recombinant human KAT3B (EP300) (532-1153, contains the catalytic domain) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.

Purity: >80%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: KAT3B Protein is suitable for use in Western Blot. Expect a band approximately ~120 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Buffer: KAT3B Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: EP300 (EP300 Products)

Background:

Synonyms: KAT3B, EP300, p300, Histone acetyltransferase p300

Background: KAT3B (also known as EP300) encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein that functions as a histone acetyltransferase. KAT3B regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. KAT3B binding is a highly accurate process and can be used for identifying enhancers and their associated activities (1). KAT3B is also useful in the study of the role of tissue-specific enhancers in human biology and diseases on a genome wide scale. Defects in KAT3B gene are a cause of Rubinstein-Taybi syndrome that play a important role in epithelial cancer and act as a classic tumor suppressor gene (2). KAT3B Protein is ideal for investigators involved in Signaling Proteins, Acetyl/Methyltransferase Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, and PKA/PKC Pathway research.

NCBI Accession: NM_001429

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size