p300 Protein (DYKDDDDK Tag)

Catalog No. ABIN2720219

Product Details

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Purification tag / Conjugate: This p300 protein is labelled with DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human EP300 / P300 (C-term DDK tag) protein expressed in Sf9 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 20 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Martinez, Abe, Hong, Molyneux, Yarnell, Löhr, Driever, Acosta, Arcos-Burgos, Muenke: "An Ultraconserved Brain-Specific Enhancer Within ADGRL3 (LPHN3) Underpins Attention-Deficit/Hyperactivity Disorder Susceptibility." in: Biological psychiatry, Vol. 80, Issue 12, pp. 943-954, (2016) (PubMed).

Target Details

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: Ep300,p300 (EP300 Products)

Synonyms: KAT3B Protein, RSTS2 Protein, p300 Protein, A430090G16 Protein, A730011L11 Protein, p300 HAT Protein, E1A binding protein p300 Protein, histone acetyltransferase p300 Protein, EP300 Protein, LOC446056 Protein, ep300 Protein, Ep300 Protein

Background: This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.

Molecular Weight: 75 kDa

NCBI Accession: NP_001420

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size