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p300 Protein (GST tag)

EP300 Origin: Human Host: Insect cells (Sf9) Recombinant >90% WB
Rockland
Catalog No. ABIN6699787
Supplier Product No.: 009-001-s63s
  • Target See all p300 (EP300) Proteins
    p300 (EP300) (E1A Binding Protein P300 (EP300))
    Protein Type
    Recombinant
    Origin
    • 6
    • 1
    • 1
    Human
    Source
    • 5
    • 2
    • 1
    Insect cells (Sf9)
    Purification tag / Conjugate
    This p300 protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-s63s
    Supplier
    Rockland
    Purpose
    P300 recombinant protein-GST fusion protein
    Purification
    Recombinant human P300 (1283-1673) was expressed in Sf9 cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
    Purity
    >90%
    Top Product
    Discover our top product EP300 Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: P300 Protein is suitable for use in Western Blot. Expect a band approximately ~74 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 μg/μL
    Buffer
    P300 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    p300 (EP300) (E1A Binding Protein P300 (EP300))
    Alternative Name
    EP300 (EP300 Products)
    Background

    Synonyms: EP300, KAT3B, Histone acetyltransferase p300

    Background: P300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein (1). P300 is related by sequence to CBP and like CBP can stimulate transcription through activation of CREB. The P300 activity is specifically inhibited by the adenovirus oncoprotein E1A. P300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. P300 is a components of DRAF1 (double-stranded RNA-activated factor-1), a positive regulator of interferon-stimulated gene transcription that functions as a direct response to viral infection (2). P300 Protein is ideal for investigators involved in Signaling Proteins, Acetyl/Methyltransferase Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, ERK/MAPK Pathway, Inflammation, Invasion/Metastasis, Metabolic Disorder, Neurobiology, NfkB Pathway, and PKA/PKC Pathway research.

    NCBI Accession
    NM_001429
    Pathways
    p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size
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