FADD Protein (His tag)

Catalog No. ABIN6699826

Supplier Product No.: 009-001-r92s

This Recombinant FADD protein is produced in Escherichia coli (E. coli).

Quick Overview for FADD Protein (His tag) (ABIN6699826)

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Western Blotting (WB)

Purity: >95%

Product Details

Purification tag / Conjugate: This FADD protein is labelled with His tag.

Supplier Product No.: 009-001-r92s

Supplier: Rockland

Purpose: FADD recombinant protein-HIS Epitope

Purification: Recombinant full-length human FADD was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.

Application Details

Application Notes:

Application Note: FADD Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. FADD Protein is suitable for use in Western Blot. Expect a band approximately ~ 27 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: FADD Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Alternative Name: FADD

Background:

Synonyms: GIG3, MORT1, MGC8528, FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD

Background: FADD or Fas-Associated protein with Death Domain is an adaptor molecule that mediates death signaling by the Fas-receptor, tumor necrosis factor receptor and TRAIL-receptor. FADD binds to these receptors via the C-terminus Death Domain which then unmasks the N-terminal effector domain of FADD thereby allowing it to recruit caspase-8 and activate the cysteine protease cascade leading to apoptosis (1). Cells lacking FADD are defective in intracellular double-stranded RNA (dsRNA)-activated gene expression, including production of type I (alpha/beta) interferons and are thus very susceptible to viral infection (2). FADD Protein is ideal for investigators involved in Signaling Proteins, Adaptor Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, and NfkB Pathway research.

NCBI Accession: NM_003824

Pathways: Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades