FADD Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2720681

Product Details

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FADD protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human FADD protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: FADD (Fas (TNFRSF6)-Associated Via Death Domain (FADD))

Alternative Name: Fadd (FADD Products)

Synonyms: MORT1 Protein, Mort1/FADD Protein, Mort1 Protein, TNFRSF6 Protein, adaptor Protein, fadd Protein, mort1 Protein, xfadd Protein, Fas associated via death domain Protein, Fas (TNFRSF6)-associated via death domain Protein, Fas associated via death domain S homeolog Protein, Fas (tnfrsf6)-associated via death domain Protein, FADD Protein, Fadd Protein, fadd.S Protein, fadd Protein

Background: The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Molecular Weight: 23.1 kDa

NCBI Accession: NP_003815

Pathways: Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades