IGF1 Protein

Catalog No. ABIN6699966

Supplier Product No.: 010-001-v21-0010

Recombinant IGF1 protein expressed in Escherichia coli (E. coli).

Quick Overview for IGF1 Protein (ABIN6699966)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: 98,00%

Product Details

Supplier Product No.: 010-001-v21-0010

Supplier: Rockland

Purpose: Mouse Insulin-like Growth Factor I Recombinant Protein

Purification: Insulin-like Growth Factor I purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent proliferation of FDC-P1 cells and is typically less than 20 ng/mL.

Application Details

Application Notes:

Application Note: Insulin-like Growth Factor I Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Insulin-like Growth Factor I in immunological assays.

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: Igf1

Background:

Synonyms: Somatamedin C, mechano growth factor, IGF-IA

Background: Insulin-like Growth Factor I, IGF-I, is a growth factor produced in response to growth hormone-stimulated liver and can be found circulating throughout the body and throughout life. IGF-I activates the IGF-I receptor (IGF1R) and the insulin receptor to mediate growth of almost every cell of the body. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Mature human IGF-I is 100 % homologous with bovine and porcine proteins. Recombinant mouse IGF-I is a non-glycosylated protein, containing 70 amino acids, with a molecular weight of 7.6 kDa.

UniProt: Q8CAR0

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation